Disinfection protocol for in vitro establishment of banana apical merystema Musa spp.

Authors

  • Yerutí Mongelós Franco Centro Multidisciplinario de Investigaciones Tecnológicas, Universidad Nacional de Asunción
  • Carlos Emilio Mussi Cataldi CEMIT-UNA
  • Nabila Nahir Duarte Ovejero
  • Maura Isabel Díaz Lezcano FCA-UNA

Abstract

The in vitro propagation of banana (Musa sp.) constitutes a profitable and safe alternative for the production of pathogen-free seedlings. One of the main problems of the in vitro establishment of this culture is the high load of contaminants that accompanies the explants. Therefore, the objective of this study was to evaluate two concentrations of Sodium hypochlorite (NaClO) for the disinfection of meristems of Musa sp. The concentrations used were 5% and 10%, with an immersion time of 5 minutes. The percentages of survival, contamination and oxidation were evaluated. There was a 100% survival of explants and 0% contamination in the treatment with 10% NaClO for 5 minutes, in contrast to 62.5% of viable explants and 37.5% contamination with fungi and bacteria in treatment with 5% NaClO. The oxidation percentage of explants was 100% in both treatments. Treatment with 10% NaClO for 5 minutes was the most effective for disinfection and in vitro establishment of the apical meristem of Musa sp.

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Published

2020-12-31

How to Cite

Mongelós Franco, Y., Mussi Cataldi, C. E., Duarte Ovejero, N. N., & Díaz Lezcano, M. I. (2020). Disinfection protocol for in vitro establishment of banana apical merystema Musa spp. CEDAMAZ, 10(2), 47–50. Retrieved from https://revistas.unl.edu.ec/index.php/cedamaz/article/view/815

Issue

Vol. 10 No. 2 (2020): CEDAMAZ

Section

Research Articles

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