First report of Leishmania naiffi in Zamora Chinchipe (Ecuador) using the gene encoding the HSP70 protein
Keywords:
Leishmania naiffi, HSP70, Leishmaniasis, Zamora Chinchipe, EcuadorAbstract
In Ecuador some of the Leishmania species belonging to the subgenus Viannia (V.) are found, causing diseases such as cutaneous, diffuse non-curative cutaneous and mucocutaneous leishmaniasis. The aim of this work was to molecularly diagnose possible cases of cutaneous leishmaniasis, from samples obtained by scraping for microscopy and lymph aspiration for culture, in Palanda and Chinchipe cantons of the Zamora Chinchipe province. Extraction of DNA from the samples and amplification of the gene encoding the HSP70 protein were performed using the Polymerase Chain Reaction (PCR). 1422 bp amplicons were obtained, which were purified and sent to Macrogen Inc., South Korea, for sequencing. The sequences presented 91% identity with Leishmania naiffi, which constitutes the first report of this species for the Zamora Chinchipe province.Metrics
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