Actividad antimicótica del aceite esencial de Plumbago scandens L.(Plumbaginaceae)

Autores/as

  • Luis Alberto Morocho-Yaguana Carrera de Laboratorio Clínico, Universidad Nacional de Loja. Loja, Ecuador
  • Anabel Marisol Carrión-Lliguín Carrera de Laboratorio Clínico, Universidad Nacional de Loja. Loja, Ecuador
  • Grace del Pilar Cambizaca-Mora Carrera de Enfermería, Universidad Técnica Particular de Loja. Loja, Ecuador
  • Humberto Daniel Riascos-Jaramillo Carrera de Laboratorio Clínico, Universidad Nacional de Loja. Loja, Ecuador

Palabras clave:

Actividad antimicótica, Aceite esencial, Plumbago scandens, Método Kirby Bauer

Resumen

Los hongos son organismos eucariotas, heterótrofos, carentes de clorofila, causantes de infecciones en los seres humanos cuando ingresan por vía respiratoria, inoculación, pinchazos o contacto físico. La incidencia de las infecciones fúngicas oportunistas está en aumento, debido al incremento de la población inmunocomprometida, el turismo y la falla terapéutica. En el presente estudio se planteó evaluar la actividad antimicótica del aceite esencial de las partes aéreas de Plumbago scandens L. extraído por arrastre de vapor y purificado por cromatografía de columna contra cepas de hongos de la American Type Culture Collection (ATCC). La actividad antimicótica se evaluó mediante la técnica de Kirby Bauer modificada; para ello se prepararon tres diluciones en acetona, en concentraciones de 50, 25 y 12,5 µg/mL, con 10 µL de estas soluciones se impregnaron discos de papel Whatman Nº 3, de 6 mm de diámetro, con un contenido de 0,5 µg, 0,25 µg y 0,125 µg/disco. Como controles positivo y negativo se usó nistatina y acetona respectivamente. La actividad antimicótica se evaluó midiendo el diámetro en mm de los halos de inhibición alrededor del disco de papel. El aceite esencial presentó actividad antimicótica contra Candida albicans (ATCC 26790), Cryptococcus neoformans (ATCC 14116), Aspergillus brasiliensis (ATCC 16404) y Trichophytum rubrum (ATCC 28188) en todas las concentraciones, excepto Candida albicans a la concentración de 0,125 µg/disco.

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Publicado

2021-07-15

Cómo citar

Morocho-Yaguana, L. A., Carrión-Lliguín, A. M., Cambizaca-Mora, G. del P., & Riascos-Jaramillo, H. D. (2021). Actividad antimicótica del aceite esencial de Plumbago scandens L.(Plumbaginaceae). CEDAMAZ, 11(1), 6–12. Recuperado a partir de https://revistas.unl.edu.ec/index.php/cedamaz/article/view/1030

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Ciencias forestales, biodiversidad y medio ambiente

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