Establishment of culture and maintenance protocols for Acanthamoeba castellanii
DOI:
https://doi.org/10.54753/cedamaz.v14i2.1837Keywords:
Acanthamoeba castellanii, Escherichia coli, axenic cultures, PYG mediumAbstract
The genus Acanthamoeba encompasses various species of free-living amoebas, often isolated from different environmental sources such as water, soil, and air. Several species are known to cause infections and diseases in both humans and animals. Additionally, amoebas like Acanthamoeba castellanii are recognized as significant reservoirs of viruses, providing protection against adverse environmental conditions, especially nucleocytoplasmic large DNA viruses, also known as giant viruses. These viruses can be isolated by directly inoculating Acanthamoeba castellanii cultures with water samples from lacustrine bodies. This study focused on establishing laboratory cultivation protocols for Acanthamoeba castellanii ATCC 30010, aiming to better understand the response of these amoebas to the environment and their interactions with protozoan predators. We developed and implemented an approach to assess the viability of this genus in a liquid medium of protease-peptone-glucose and a non-nutritive solid medium, using Escherichia coli ATCC 25922 as a substrate. Incubation at specific temperatures and regular maintenance allowed for the establishment of axenic cultures of Acanthamoeba castellanii ATCC 30010. Through observation under an inverted microscope (10x and 40x), the growth of Acanthamoeba was verified, confirming the trophozoite state of the cells and the presence of the amoebal vacuole in both types of culture.References
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