Influence of sucrose on in vitro germination of Cattleya maxima Lindl
DOI:
https://doi.org/10.54753/cedamaz.v14i1.2041Keywords:
Orchids, Capsules, In vitro germination, ConservationAbstract
The Orchidaceae family has great ornamental importance, due to the beauty of its flowers, with Cattleya maxima Lindl., standing out as a highly commercialized species, since it has large flowers and very attractive bright colors. The species is threatened by indiscriminate extraction, destruction of natural habitat, climate change and limited capsule production and a low germination rate. Due to orchid seeds’ difficulty in germinating naturally, asymbiotic germination methodologies have been developed under in vitro conditions. Therefore, the objective of the present study was to contribute to generating scientific information related to the influence of sucrose on the in vitro asymbiotic germination of C. maxima seeds, from physiologically mature capsules, obtained from a germplasm collection, the sedes were obtained and under aseptic conditions, they were inoculated in vitro in the Knudson-C (1946) culture medium, supplemented with myo-inositol, thiamine, pyridoxine, nicotinic acid, coconut water, activated carbon, agar; and, in four sucrose concentrations (treatments). The asymbiotic germination of the seeds, testing different concentrations of sucrose, recorded high germination percentages; Thus, T0 (0 g L-1) with 91%, T1 (20 g L-1) with 87%, T2 (30 g L-1) with 86% and T3 (40 g L-1) with 74%. The sucrose concentration did not influence in vitro germination, however it can be deduced that the viability of the seeds and the chemical composition of the Knudson C (KC-1946) culture medium presented favorable conditions for the asymbiotic germination of Cattleya maxima.Metrics
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