Control of phenolic oxidation of Handroanthus heptaphyllus nodal segments under in vitro conditions
Keywords:
Explants, White-flowered lapacho, Micropropagation, Parent plants, Phenolic oxidationAbstract
In vitro culture could become a viable option in the mass production of Handroanthus heptaphyllus (Vell.) Mattos (whiteflowered lapacho), an indigenous species from Paraguay, from the family Bignoniaceae. This species possesses a unique characteristic due to its white inflorescences, which are observed in a few specimens of the genus. The objective of the present work was to establish a protocol for the control of in vitro oxidation of Handroanthus heptaphyllus nodal segments by using activated charcoal in different concentrations (1, 2, 3 and 4 g.L-1) in Murashige and Skoog culture medium. A total of 40 nodal segments were used from parent plants that were kept in the nursery. These parent plants were treated with the application of mancozeb at 3 g.L-1. The variables measured were oxidation and survival up to 35 days of incubation. Analysis of variance and Tukey test were performed with a confidence level of 5%. Statistical analyses showed significant differences in oxidation control. The 3 g.L-1 concentration of activated charcoal has been the most effective.Metrics
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